Part:BBa_K5038025
PT7-lacO-LIRA_input01-T7TE
Usage and Biology
LIRA_input01 is the target to be detected by LIRA_H01, it will bind to the loop at the top of LIRA_H01, and then change its conformation so that the ribosome could bind to the RBS.
(Figure 1. LIRA_input01 binds to LIRA_H01)
This composite part is used to express input01 in E.coli to test the function of H01.
Characterization
T7 promoter could be induced by IPTG, so the intracellularly concentration of LIRA_input01 produces a large difference between the two conditions of IPTG presence/absence.
Compared to BBa_K5038023, this plasmid has an additional lac operator downstream of T7 promoter, which is the binding site of LacI repression protein and could further prevent the transcription leakage.
This composite part is equipped by the following plasmids and co-transformed with the corresponding H01 plasmid. See BBa_K5038021 for details.
(Table 1. LIRA_input 01 plasmids and the corresponding H01 plasmid)
| No. | LIRA_input01 plasmids | corresponding H01 plasmid |
|---|---|---|
| 1 | pCDFDuet-lacO-input01 | pET-15b-lacO-H01-linker-EGFP |
| 2 | pCOLADuet-lacO-input01 | pET-15b-lacO-H01-linker-EGFP |
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
| None |